быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
NCAM /CD56 Rabbit mAb (A23300)
- Описание
- Характеристики
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Overview
Product name NCAM /CD56 Rabbit mAb Catalog No. A23300 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC60182 Background
Predicted to enable LRR domain binding activity and phosphatase binding activity. Involved in commissural neuron axon guidance and regulation of semaphorin-plexin signaling pathway. Acts upstream of or within several processes, including homotypic cell-cell adhesion; positive regulation of calcium-mediated signaling; and regulation of exocyst assembly. Located in several cellular components, including external side of plasma membrane; growth cone; and neuronal cell body. Is expressed in several structures, including embryo mesenchyme; nervous system; sensory organ; skin; and urinary system. Human ortholog(s) of this gene implicated in bipolar disorder; middle cerebral artery infarction; and pancreatic cancer. Orthologous to human NCAM1 (neural cell adhesion molecule 1).Immunogen information
Immunogen Recombinant protein of mouse NCAM /CD56. Sequence Email for sequence Gene ID Swiss Prot Synonyms CD56; Ncam; E-NCAM; NCAM-1 Calculated MW 67kDa/80kDa/93kDa/119kDa Observed MW 120-220kDa Applications
Reactivity Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples Neuro-2a, Mouse brain, Rat brain Cellular location Cell membrane ; Single-pass type I membrane protein 
Western blot analysis of various lysates, using NCAM /CD56 Rabbit mAb (A23300) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded mouse brain using NCAM /CD56 Rabbit mAb (A23300) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat brain using NCAM /CD56 Rabbit mAb (A23300) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat colon using NCAM /CD56 Rabbit mAb (A23300) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of Neuro-2a and L-929 cells using NCAM /CD56 Rabbit mAb (A23300) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat brain cells using NCAM /CD56 Rabbit mAb (A23300) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Мышь, Крыса Immunogen Recombinant protein of mouse NCAM /CD56. Isotype IgG