быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303)
- Описание
- Характеристики
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Overview
Product name PI3 Kinase p85 alpha/beta Rabbit PolymAb® Catalog No. A23303 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC57210_ARC55272 Background
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]Immunogen information
Immunogen A synthesized peptide derived from human PI3 Kinase p85 alpha/beta. Sequence Email for sequence Gene ID Swiss Prot Synonyms PIK3R1/PIK3R2 Calculated MW 42-54kDa/81-84kDa Observed MW 85kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:2000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples Cellular location cell-cell junction, cis-Golgi network, cytoplasm, cytosol, nucleus, perinuclear region of cytoplasm, plasma membrane 
Western blot analysis of various lysates, using PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot analysis of Rat lung, using PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded human kidney using PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney using PI3 Kinase p85 alpha/beta Rabbit PolymAb® (A23303) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthesized peptide derived from human PI3 Kinase p85 alpha/beta. Isotype IgG