быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
MGLL Rabbit PolymAb® (A23306)
- Описание
- Характеристики
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Overview
Product name MGLL Rabbit PolymAb® Catalog No. A23306 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58998_ARC58991 Background
This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.Immunogen information
Immunogen Recombinant protein of human MGLL. Sequence Email for sequence Gene ID Swiss Prot Synonyms MGL; HUK5; MAGL; HU-K5 Calculated MW 33kDa Observed MW 33kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples U-87MG, Mouse brain, Rat testis Cellular location Cytoplasm, Membrane, Peripheral membrane protein, cytosol 
Western blot analysis of various lysates, using MGLL Rabbit PolymAb® (A23306) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded human liver using MGLL Rabbit PolymAb® (A23306) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney using MGLL Rabbit PolymAb® (A23306) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of rat kidney cells using MGLL Rabbit PolymAb® (A23306) at dilution of 1:50 (40x lens).Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human MGLL. Isotype IgG