быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Citrate synthetase Rabbit mAb (A23371)
- Описание
- Характеристики
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Overview
Product name Citrate synthetase Rabbit mAb Catalog No. A23371 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59586 Background
The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found.Immunogen information
Immunogen Recombinant protein of human Citrate synthetase Sequence Email for sequence Gene ID Swiss Prot Synonyms CS; citrate synthase Calculated MW 52kDa Observed MW 51kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:1000 - 1:5000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples HeLa, 293F, NIH/3T3, Mouse heart, Rat brain Cellular location Mitochondrion matrix 
Western blot analysis of various lysates, using Citrate synthetase Rabbit mAb (A23371) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded human kidney using Citrate synthetase Rabbit mAb (A23371) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C2C12 cells using Citrate synthetase Rabbit mAb (A23371) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human Citrate synthetase Isotype IgG