быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
FAM38A/PIEZO1 Rabbit mAb (A23380)
- Описание
- Характеристики
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Overview
Product name FAM38A/PIEZO1 Rabbit mAb Catalog No. A23380 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC60118 Background
The protein encoded by this gene is a mechanically-activated ion channel that links mechanical forces to biological signals. The encoded protein contains 36 transmembrane domains and functions as a homotetramer. Defects in this gene have been associated with dehydrated hereditary stomatocytosis.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 2199-2429 of human PIEZO1(NP_001136336.2). Sequence VVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIIPFTAQAYEELSRQFDPQPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKRELYNGTADITLRFTWNFQRDLAKGGTVEYANEKHMLALAPNSTARRQLASLLEGTSDQSVVIPNLFPKYIRAPNGPEANPVKQLQPNEEADYLGVRIQLRREQGAGATGFLEWWVIELQECRTDCNLLPMVIFSDKVSP Gene ID Swiss Prot Synonyms ER; DHS; Mib; LMPH3; FAM38A; LMPHM6 Calculated MW 287kDa Observed MW 287kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples MCF7, Mouse lung Cellular location Cell membrane, Cell projection, Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Multi-pass membrane protein, lamellipodium membrane Customer validation IHC(Mus musculus)
Western blot analysis of MCF7, using FAM38A/PIEZO1 Rabbit mAb (A23380) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
Western blot analysis of Mouse lung, using FAM38A/PIEZO1 Rabbit mAb (A23380) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.
Immunohistochemistry analysis of paraffin-embedded human kidney using FAM38A/PIEZO1 Rabbit mAb (A23380) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using FAM38A/PIEZO1 Rabbit mAb (A23380) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat liver using FAM38A/PIEZO1 Rabbit mAb (A23380) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 2199-2429 of human PIEZO1(NP_001136336.2). Isotype IgG