быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Orexin B Rabbit mAb (A23383)
- Описание
- Характеристики
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Overview
Product name Orexin B Rabbit mAb Catalog No. A23383 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC60837 Background
This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.Immunogen information
Immunogen Recombinant protein of human Orexin B/Hypocretin-2 Sequence Email for sequence Gene ID Swiss Prot Synonyms OX; PPOX; NRCLP1 Calculated MW 13kDa Observed MW 32kDa Applications
Reactivity Human Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293F-Orexin B Cellular location Cell junction, Cytoplasmic vesicle, Rough endoplasmic reticulum, synapse 
Western blot analysis of 293F-Orexin B, using Orexin B Rabbit mAb (A23383) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of 293F-Orexin B and 293F using Orexin B Rabbit mAb (A23383) at dilution of 1:100 (40x lens).Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of human Orexin B/Hypocretin-2 Isotype IgG