быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
MDM2 Rabbit mAb (A23388)
- Описание
- Характеристики
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Overview
Product name MDM2 Rabbit mAb Catalog No. A23388 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC60296 Background
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.Immunogen information
Immunogen Recombinant protein of human MDM2. Sequence CVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLA Gene ID Swiss Prot Synonyms HDMX; LSKB; hdm2; ACTFS Calculated MW 55kDa Observed MW 90kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:1000 - 1:5000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples U2OS Cellular location Cytoplasm, Nucleus, nucleolus, nucleoplasm 
Western blot analysis of U2OS, using MDM2 Rabbit mAb (A23388) at 1:2000 dilution.U2OS cells were treated by Nutlin 3a(10μM ) at 37℃ for 24 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of human MDM2. Isotype IgG