быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Sestrin 2 Rabbit mAb (A23685)
- Описание
- Характеристики
-
Overview
Product name Sestrin 2 Rabbit mAb Catalog No. A23685 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC61316 Background
This gene encodes a member of the sestrin family of PA26-related proteins. The encoded protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions.Immunogen information
Immunogen Recombinant protein of human Sestrin 2 Sequence Email for sequence Gene ID Swiss Prot Synonyms HI95; SES2; SEST2 Calculated MW 54kDa Observed MW 60kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:1000 - 1:5000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, Raw264.7, NIH-3T3 Cellular location cytoplasm 
Western blot analysis of extracts of various lysates, using Sestrin 2 Rabbit mAb (A23685) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of extracts of various lysates, using Sestrin 2 Rabbit mAb (A23685) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human Sestrin 2 Isotype IgG