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Размер
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PE Rabbit anti-Mouse CD56/NCAM mAb (A23749)
- Описание
- Характеристики
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Overview
Product name PE Rabbit anti-Mouse CD56/NCAM mAb Catalog No. A23749 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC60214-PE Background
Predicted to enable LRR domain binding activity and phosphatase binding activity. Involved in commissural neuron axon guidance and regulation of semaphorin-plexin signaling pathway. Acts upstream of or within several processes, including homotypic cell-cell adhesion; positive regulation of calcium-mediated signaling; and regulation of exocyst assembly. Located in several cellular components, including external side of plasma membrane; growth cone; and neuronal cell body. Is expressed in several structures, including embryo mesenchyme; nervous system; sensory organ; skin; and urinary system. Human ortholog(s) of this gene implicated in bipolar disorder; middle cerebral artery infarction; and pancreatic cancer. Orthologous to human NCAM1 (neural cell adhesion molecule 1).Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 20-711 of mouse CD56/NCAM (NP_001106675.1). Sequence LQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRISVVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDKNDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKTLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEFQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEESWHFKWYDAKEANMEGIVTIMGLKPETRYSVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTAGLST Gene ID Swiss Prot Synonyms CD56; Ncam; E-NCAM; NCAM-1 Calculated MW 67KDa/80KDa/93KDa/119KDa Observed MW Applications
Reactivity Mouse Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3Key application Flow Cytometry Positive samples Cellular location Plama membrane, extracellular, cytosol 
Flow cytometry: 1X10^6 L-929 cells(negative control, left) and Neuro-2a cells (right) were surface-stained with PE Rabbit anti-Mouse CD56/NCAM mAb(A23749, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 Neuro-2a cells were surface-stained with PE Rabbit IgG isotype control (5 μl/Test, left) or PE Rabbit anti-Mouse CD56/NCAM mAb(A23749, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 20-711 of mouse CD56/NCAM (NP_001106675.1). Isotype IgG