быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospholamban Rabbit mAb (A23750)
- Описание
- Характеристики
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Overview
Product name Phospholamban Rabbit mAb Catalog No. A23750 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59796 Background
The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy.Immunogen information
Immunogen A synthesized peptide derived from human Phospholamban. Sequence Email for sequence Gene ID Swiss Prot Synonyms PLN; CMD1P; CMH18; PLB; phospholamban Calculated MW 6kDa Observed MW 12kDa/24kDa Applications
Reactivity Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples Mouse heart, Rat heart Cellular location 
Western blot analysis of various lysates, using Phospholamban Rabbit mAb (A23750) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of mouse heart using Phospholamban Rabbit mAb (A23750) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of rat heart using Phospholamban Rabbit mAb (A23750) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Мышь, Крыса Immunogen A synthesized peptide derived from human Phospholamban. Isotype IgG