быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
KLHDC2 Rabbit mAb (A23751)
- Описание
- Характеристики
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Overview
Product name KLHDC2 Rabbit mAb Catalog No. A23751 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC60813 Background
Enables ubiquitin ligase-substrate adaptor activity. Involved in ubiquitin-dependent protein catabolic process via the C-end degron rule pathway. Located in nuclear body and nuclear membrane. Is active in Cul2-RING ubiquitin ligase complex and nucleus.Immunogen information
Immunogen A synthesized peptide derived from human KLHDC2. Sequence Email for sequence Gene ID Swiss Prot Synonyms KLHDC2; HCLP-1; HCLP1; LCP; kelch domain containing 2 Calculated MW 28kDa/46kDa Observed MW 46kDa Applications
Reactivity Human, Mouse Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples PC-3, Mouse brain Cellular location Nucleus 
Western blot analysis of various lysates, using KLHDC2 Rabbit mAb (A23751) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded human kidney using KLHDC2 Rabbit mAb (A23751) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using KLHDC2 Rabbit mAb (A23751) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen A synthesized peptide derived from human KLHDC2. Isotype IgG