быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Beta-galactosidase （GLB1）Rabbit mAb (A23753)
- Описание
- Характеристики
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Overview
Product name Beta-galactosidase （GLB1）Rabbit mAb Catalog No. A23753 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC61596 Background
This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome.Immunogen information
Immunogen Recombinant protein of human Beta-galactosidase （GLB1）. Sequence Email for sequence Gene ID Swiss Prot Synonyms GLB1; EBP; ELNR1; MPS4B; beta-galactosidase Calculated MW 60kDa/72kDa/76kDa Observed MW 76kDa Applications
Reactivity Human, Rat Tested applications WB IHC-P Recommended dilution - WB 1:1000 - 1:5000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HCT116, 293F Cellular location Cytoplasm, Lysosome, perinuclear region 
Western blot analysis of various lysates, using Beta-galactosidase （GLB1）Rabbit mAb (A23753) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded human liver using Beta-galactosidase （GLB1）Rabbit mAb (A23753) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Beta-galactosidase （GLB1）Rabbit mAb (A23753) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Крыса Immunogen Recombinant protein of human Beta-galactosidase （GLB1）. Isotype IgG