быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CD73/NT5E Rabbit mAb (A23754)
- Описание
- Характеристики
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Overview
Product name CD73/NT5E Rabbit mAb Catalog No. A23754 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC56899 Background
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen A synthesized peptide derived from human CD73/NT5E. Sequence Email for sequence Gene ID Swiss Prot Synonyms NT5E; CALJA; CD73; E5NT; NT; NT5; NTE; eN; eNT; 5'-nucleotidase Calculated MW 57kDa/63kDa Observed MW 70kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HCT116 Cellular location Cell membrane, GPI-anchor, Lipid-anchor 
Western blot analysis of various lysates, using CD73/NT5E Rabbit mAb (A23754) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 45s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthesized peptide derived from human CD73/NT5E. Isotype IgG