быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
SLC25A13/Citrin Rabbit mAb (A23756)
- Описание
- Характеристики
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Overview
Product name SLC25A13/Citrin Rabbit mAb Catalog No. A23756 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC61350 Background
This gene is a member of the mitochondrial carrier family. The encoded protein contains four EF-hand Ca(2+) binding motifs in the N-terminal domain, and localizes to mitochondria. The protein catalyzes the exchange of aspartate for glutamate and a proton across the inner mitochondrial membrane, and is stimulated by calcium on the external side of the inner mitochondrial membrane. Mutations in this gene result in citrullinemia, type II. Multiple transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant protein of human SLC25A13/Citrin. Sequence Email for sequence Gene ID Swiss Prot Synonyms SLC25A13; ARALAR2; CITRIN; CTLN2; solute carrier family 25 member 13 Calculated MW 74kDa Observed MW 74kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:1000 - 1:5000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples A-431, Hep G2, Mouse liver, Mouse kidney, Rat liver Cellular location Mitochondrion inner membrane, Multi-pass membrane protein 
Western blot analysis of various lysates, using SLC25A13/Citrin Rabbit mAb (A23756) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of various lysates, using SLC25A13/Citrin Rabbit mAb (A23756) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.
Immunohistochemistry analysis of paraffin-embedded human tonsil using SLC25A13/Citrin Rabbit mAb (A23756) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse spleen using SLC25A13/Citrin Rabbit mAb (A23756) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat spleen using SLC25A13/Citrin Rabbit mAb (A23756) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human SLC25A13/Citrin. Isotype IgG