быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
EGFR Rabbit mAb (A23764)
- Описание
- Характеристики
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Overview
Product name EGFR Rabbit mAb Catalog No. A23764 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC61438 Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.Immunogen information
Immunogen Recombinant protein of human EGFR. Sequence Email for sequence Gene ID Swiss Prot Synonyms EGFR; ERBB; ERBB1; HER1; NISBD2; PIG61; mENA; epidermal growth factor receptor Calculated MW 44kDa/69kDa/77kDa/134kDa Observed MW 145-175kDa Applications
Reactivity Human Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples A431 cells Cellular location Cell membrane, Single-pass type I membrane protein 
Western blot analysis of A-431, using EGFR Rabbit mAb (A23764) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.
Immunohistochemistry of paraffin-embedded human lung squamous carcinoma tissue using EGFR Rabbit mAb (A23764) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human placenta using EGFR Rabbit mAb (A23764) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human urothelial carcinoma using EGFR Rabbit mAb (A23764) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of human EGFR. KO Validated Validated Isotype IgG