быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
AP2B1 Rabbit mAb (A23784)
- Описание
- Характеристики
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Overview
Product name AP2B1 Rabbit mAb Catalog No. A23784 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC61904 Background
The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant protein of human AP2B1. Sequence Email for sequence Gene ID Swiss Prot Synonyms AP2B1; ADTB2; AP105B; AP2-BETA; CLAPB1; AP-2 complex subunit beta Calculated MW 98kDa/104kDa/105kDa Observed MW 105kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples Hela, Mouse lung, Rat testis Cellular location Cell membrane, Cytoplasmic side, Membrane, Peripheral membrane protein, coated pit 
Western blot analysis of various lysates, using AP2B1 Rabbit mAb (A23784) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human AP2B1. Isotype IgG