быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
SDHB Rabbit mAb (A23832)
- Описание
- Характеристики
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Overview
Product name SDHB Rabbit mAb Catalog No. A23832 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC61606 Background
Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 29-280 of human SDHB (P21912). Sequence AQTAAATAPRIKKFAIYRWDPDKAGDKPHMQTYEVDLNKCGPMVLDALIKIKNEVDSTLTFRRSCREGICGSCAMNINGGNTLACTRRIDTNLNKVSKIYPLPHMYVIKDLVPDLSNFYAQYKSIEPYLKKKDESQEGKQQYLQSIEEREKLDGLYECILCACCSTSCPSYWWNGDKYLGPAVLMQAYRWMIDSRDDFTEERLAKLQDPFSLYRCHTIMNCTRTCPKGLNPGKAIAEIKKMMATYKEKKASV Gene ID Swiss Prot Synonyms SDHB; CWS2; IP; PGL4; SDH; SDH1; SDH2; SDHIP; succinate dehydrogenase complex iron sulfur subunit B Calculated MW 31kDa Observed MW 32kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:2000 - 1:4000
- IHC-P 1:50 - 1:200
- IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples SK-MEL-28, K-562, 293T, Mouse heart, Rat heart Cellular location Matrix side, Mitochondrion inner membrane, Peripheral membrane protein 
Western blot analysis of various lysates, using SDHB Rabbit mAb (A23832) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.
Immunohistochemistry analysis of paraffin-embedded Human gastrointestinal stromal tumor using SDHB Rabbit mAb (A23832) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of mouse heart using SDHB Rabbit mAb (A23832) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 29-280 of human SDHB (P21912). KO Validated Validated Isotype IgG