быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Connexin 43 Rabbit PolymAb® (A23928)
- Описание
- Характеристики
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Overview
Product name Connexin 43 Rabbit PolymAb® Catalog No. A23928 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58819_ARC58810 Background
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations.Immunogen information
Immunogen Recombinant protein of human Connexin 43. Sequence Email for sequence Gene ID Swiss Prot Synonyms HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; EKVP3; HLHS1; PPKCA Calculated MW 43kDa Observed MW Refer to figures Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:1000 - 1:5000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples U-251MG, A-549, Rat brain Cellular location Cell junction, Cell membrane, Endoplasmic reticulum, Multi-pass membrane protein, gap junction 
Western blot analysis of extracts from Rat brain , using Connexin 43 Rabbit PolymAb® (A23928) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Western blot analysis of various lysates , using Connexin 43 Rabbit PolymAb® (A23928) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of mouse heart using Connexin 43 Rabbit PolymAb® (A23928) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of rat heart using Connexin 43 Rabbit PolymAb® (A23928) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human Connexin 43. Isotype IgG