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Размер
- 100 μL
- 200 μL
- 50 μL
Semaphorin-4D/CD100 Rabbit mAb (A24404)
- Описание
- Характеристики
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Overview
Product name Semaphorin-4D/CD100 Rabbit mAb Catalog No. A24404 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC63157 Background
Plexin-B1 is related to axon guidance in the central nervous system. The Sema4D function is also widely detected in the immune system and was found to be the first semaphorin expressed on the surface of many types of immune cells. In the immune system, CD72 is a low-affinity receptor for Sema4D, and studies have shown that Sema4D can not only regulate T cell activation, but also participate in the regulation of B cell survival and differentiation. In the immune system, soluble fragments containing extracellular domains produced by proteolytic cleavage can regulate many physiological functions of Sema4D. Sema4D is also associated with tumorigenesis because studies have confirmed that it is overexpressed in various types of solid tumor cells. To some extent, the role of Sema4D in tumorigenesis is related to its ability to cause tumor angiogenesis, cell invasion, and immunosuppression by enhancing bone marrow-derived suppressor cell function.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 22-734 of human Semaphorin-4D/CD100(NP_006369.3). Sequence MAFAPIPRITWEHREVHLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVFAVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSATSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSPDSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKWTSFLKARLICSRPDSGLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVGLSAVCAYNLSTAEEVFSHGKYMQSTTVEQSHTKWVRYNGPVPKPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYTQIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFEPVQTLLLSSKKGNRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCAWSPPTATCVALHQTESPSRGLIQEMSGDASVCPDKSKGSYRQHFFKHGGTAELKCSQKSNLARVFWKFQNGVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPTPAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLHSEKTMYLKSSDNR Gene ID Swiss Prot Synonyms SEMA4D; C9orf164; CD100; M-sema-G; SEMAJ; coll-4; semaphorin-4D Calculated MW 82kDa/96kDa Observed MW Applications
Reactivity Human Tested applications FC Recommended dilution - FC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Cell membrane, Single-pass type I membrane protein 
Flow cytometry: 1X10^6 PC-3 cells (negative control, Left) and Molt-4 cells(Right) were surface-stained with Semaphorin-4D/CD100 Rabbit mAb(A24404, 2μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 2μg/mL, blue line), followed by Alexa Fluor®647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 Molt-4 cells cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or Semaphorin-4D/CD100 Rabbit mAb(A24404, 2 μg/mL, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 22-734 of human Semaphorin-4D/CD100(NP_006369.3). Isotype IgG