быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
F4/80 Rabbit mAb (A24415)
- Описание
- Характеристики
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Overview
Product name F4/80 Rabbit mAb Catalog No. A24415 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC62251 Background
Predicted to enable G protein-coupled receptor activity. Predicted to be involved in adenylate cyclase-activating G protein-coupled receptor signaling pathway. Predicted to act upstream of or within G protein-coupled receptor signaling pathway and adaptive immune response. Located in external side of plasma membrane. Is expressed in several structures, including cardiovascular system; central nervous system; genitourinary system; hemolymphoid system; and intestine. Orthologous to human ADGRE1 (adhesion G protein-coupled receptor E1).Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 367-645 of mouse F4/80(NP_034260.1). Sequence KRILFKCKEDLILQSEQIQQCQAVQGRDLGYASFCTLVNATFTILDNTCENKSAPVSLQSAATSVSLVLEQATTWFELSKEETSTLGTILLETVESTMLAALLIPSGNASQMIQTEYLDIESKVINEECKENESINLAARGDKMNVGCFIIKESVSTGAPGVAFVSFAHMESVLNERFFEDGQSFRKLRMNSRVVGGTVTGEKKEDFSKPIIYTLQHIQPKQKSERPICVSWNTDVEDGRWTPSGCEIVEASETHTVCSCNRMANLAIIMASGELTMEF Gene ID Swiss Prot Synonyms Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7 Calculated MW 102kDa Observed MW Refer to figures Applications
Reactivity Mouse Tested applications FC Recommended dilution - FC 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Cell membrane, Multi-pass membrane protein 
Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and RAW 264.7 cells (right) were surface-stained with F4/80 Rabbit mAb(A24415, 2μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 2μg/mL, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 RAW 264.7 cells cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or F4/80 Rabbit mAb(A24415, 2 μg/mL, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 367-645 of mouse F4/80(NP_034260.1). Isotype IgG