быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CD101/IGSF2 Rabbit mAb (A24427)
- Описание
- Характеристики
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Overview
Product name CD101/IGSF2 Rabbit mAb Catalog No. A24427 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC62615 Background
Predicted to enable hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides. Predicted to be involved in cell surface receptor signaling pathway. Predicted to act upstream of or within positive regulation of myeloid leukocyte differentiation. Located in extracellular exosome.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 21-410 of human CD101/IGSF2 (NP_004249.2). Sequence QREVTVQKGPLFRAEGYPVSIGCNVTGHQGPSEQHFQWSVYLPTNPTQEVQIISTKDAAFSYAVYTQRVRSGDVYVERVQGNSVLLHISKLQMKDAGEYECHTPNTDEKYYGSYSAKTNLIVIPDTLSATMSSQTLGKEEGEPLALTCEASKATAQHTHLSVTWYLTQDGGGSQATEIISLSKDFILVPGPLYTERFAASDVQLNKLGPTTFRLSIERLQSSDQGQLFCEATEWIQDPDETWMFITKKQTDQTTLRIQPAVKDFQVNITADSLFAEGKPLELVCLVVSSGRDPQLQGIWFFNGTEIAHIDAGGVLGLKNDYKERASQGELQVSKLGPKAFSLKIFSLGPEDEGAYRCVVAEVMKTRTGSWQVLQRKQSPDSHVHLRKPAA Gene ID Swiss Prot Synonyms V7; IGSF2; EWI-101 Calculated MW 115kDa Observed MW 140kDa Applications
Reactivity Human, Mouse Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293T transfected with CD101 Cellular location Plasma membrane, Extracelluar 
Western blot analysis of normal 293T cells and 293T transfected with CD101/IGSF2 protein, using CD101/IGSF2 Rabbit mAb (A24427) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunofluorescence analysis of 293F-CD101 and 293F cells using CD101/IGSF2 Rabbit mAb (A24427) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 21-410 of human CD101/IGSF2 (NP_004249.2). Isotype IgG