быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
ATF5 Rabbit mAb (A3563)
- Описание
- Характеристики
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Overview
Product name ATF5 Rabbit mAb Catalog No. A3563 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2043 Background
Enables several functions, including DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II transcription regulatory region sequence-specific DNA binding activity; and tubulin binding activity. Involved in several processes, including fat cell differentiation; regulation of cell cycle process; and regulation of transcription, DNA-templated. Located in centrosome; cytosol; and nucleoplasm.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 183-282 of human ATF5 (Q9Y2D1). Sequence PQQPPPPSPPQPSRLAPYPHPATTRGDRKQKKRDQNKSAALRYRQRKRAEGEALEGECQGLEARNRELKERAESVEREIQYVKDLLIEVYKARSQRTRSC Gene ID Swiss Prot Synonyms ATFX; HMFN0395 Calculated MW 31kDa Observed MW 35kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HeLa, Jurkat, SH-SY5Y, Mouse liver, Mouse brain, Rat lung, Rat liver, Rat brain Cellular location centrosome, cytosol, nucleoplasm, nucleus 
Western blot analysis of extracts of various cell lines, using ATF5 Rabbit mAb (A3563) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded human placenta using ATF5 Rabbit mAb (A3563) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 183-282 of human ATF5 (Q9Y2D1). Isotype IgG