быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Exportin 5 (XPO5) Rabbit mAb (A3813)
- Описание
- Характеристики
-
Overview
Product name Exportin 5 (XPO5) Rabbit mAb Catalog No. A3813 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0838 Background
This gene encodes a member of the karyopherin family that is required for the transport of small RNAs and double-stranded RNA-binding proteins from the nucleus to the cytoplasm. The encoded protein translocates cargo through the nuclear pore complex in a RanGTP-dependent process.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Exportin 5 (XPO5) (Q9HAV4). Sequence MAMDQVNALCEQLVKAVTVMMDPNSTQRYRLEALKFCEEFKEKCPICVPCGLRLAEKTQVAIVRHFGLQILEHVVKFRWNGMSRLEKVYLKNSVMELIANGTLNILEEENHIKDALSRIVVEMIKREWPQHWPDMLIELDTLSKQGETQTELVMFILLRLAEDVVTFQTLPPQRRRDIQQTLTQNMERIFSFLLNTLQEN Gene ID Swiss Prot Synonyms exp5 Calculated MW 136kDa Observed MW 136kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples U-2 OS, MCF7, U-251MG，Mouse brain, Mouse kidney, Rat brain Cellular location Cytoplasm, Nucleus 
Western blot analysis of extracts of various cell lines, using Exportin 5 (XPO5) (XPO5) Rabbit mAb (A3813) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using Exportin 5 (XPO5) (XPO5) Rabbit mAb (A3813) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Exportin 5 (XPO5) (XPO5) Rabbit mAb (A3813) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
-
Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Exportin 5 (XPO5) (Q9HAV4). Isotype IgG