быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
DKK3 Rabbit mAb (A3892)
- Описание
- Характеристики
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Overview
Product name DKK3 Rabbit mAb Catalog No. A3892 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0861 Background
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 200-350 of human DKK3 (Q9UBP4) (NP_056965.3) Sequence TRGSNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPASRLLDLITWELEPDGALDRCPCASGLLCQPHSHSLVYVCKPTFVGSRDQDGEILLPREVPDEYEVGSFMEEVRQELEDLERSLTEEMALREPAAAAAALLGGEEI Gene ID Swiss Prot Synonyms RIG; CRRL; REIC Calculated MW 38kDa Observed MW 60kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples Mouse brain, Rat brain Cellular location Extracellular 
Western blot analysis of extracts of various cell lines, using DKK3 Rabbit mAb (A3892) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Immunofluorescence analysis of SH-SY5Y cells using DKK3 Rabbit mAb (A3892) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 200-350 of human DKK3 (Q9UBP4) (NP_056965.3) Isotype IgG