быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
FAAH1 Rabbit mAb (A4099)
- Описание
- Характеристики
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Overview
Product name FAAH1 Rabbit mAb Catalog No. A4099 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0903 Background
This gene encodes a protein that is responsible for the hydrolysis of a number of primary and secondary fatty acid amides, including the neuromodulatory compounds anandamide and oleamide.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 480-579 of human FAAH1 (O00519). Sequence DLNAPGRATGAVSYTMLYNCLDFPAGVVPVTTVTAEDEAQMEHYRGYFGDIWDKMLQKGMKKSVGLPVAVQCVALPWQEELCLRFMREVERLMTPEKQSS Gene ID Swiss Prot Synonyms PSAB; FAAH-1 Calculated MW 63kDa Observed MW 63kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples Mouse liver, Mouse brain, Mouse kidney, HepG2, U-251MG, Rat liver Cellular location Cytoplasm, Endomembrane system, Single-pass membrane protein, cytoskeleton Customer validation WB(Rattus norvegicus)
Western blot analysis of extracts of various cell lines, using FAAH1 Rabbit mAb (A4099) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using FAAH1 Rabbit mAb (A4099) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of NIH-3T3 cells using FAAH1 Rabbit mAb (A4099) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 480-579 of human FAAH1 (O00519). Isotype IgG