быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Coagulation Protein C Rabbit mAb (A4532)
- Описание
- Характеристики
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Overview
Product name Coagulation Protein C Rabbit mAb Catalog No. A4532 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1023 Background
This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 300-461 of human Coagulation Coagulation Protein C (P04070). Sequence IALLHLAQPATLSQTIVPICLPDSGLAERELNQAGQETLVTGWGYHSSREKEAKRNRTFVLNFIKIPVVPHNECSEVMSNMVSENMLCAGILGDRQDACEGDSGGPMVASFHGTWFLVGLVSWGEGCGLLHNYGVYTKVSRYLDWIHGHIRDKEAPQKSWAP Gene ID Swiss Prot Synonyms PC; APC; PROC1; THPH3; THPH4 Calculated MW 52kDa Observed MW 52kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HT-29, Mouse liver Cellular location Endoplasmic reticulum, Golgi apparatus, Secreted 
Western blot analysis of extracts of various cell lines, using Coagulation Coagulation Protein C Rabbit mAb (A4532) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
Immunofluorescence analysis of mouse liver using Coagulation Coagulation Protein C Rabbit mAb (A4532) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 300-461 of human Coagulation Coagulation Protein C (P04070). Isotype IgG