быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CDC37 Rabbit mAb (A4582)
- Описание
- Характеристики
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Overview
Product name CDC37 Rabbit mAb Catalog No. A4582 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1040 Background
The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CDC37 (Q16543). Sequence MVDYSVWDHIEVSDDEDETHPNIDTASLFRWRHQARVERMEQFQKEKEELDRGCRECKRKVAECQRKLKELEVAEGGKAELERLQAEAQQLRKEERSWEQ Gene ID Swiss Prot Synonyms P50CDC37 Calculated MW 44kDa Observed MW 50kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, 293T, Jurkat, Mouse brain, Mouse spleen, Rat lung, Rat spleen Cellular location Cytoplasm 
Western blot analysis of extracts of various cell lines, using CDC37 Rabbit mAb (A4582) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CDC37 (Q16543). Isotype IgG