быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
IRAK2 Rabbit mAb (A4655)
- Описание
- Характеристики
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Overview
Product name IRAK2 Rabbit mAb Catalog No. A4655 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1078 Background
IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 50-250 of human IRAK2 (O43187). Sequence RVQGVSITRELLWWWGMRQATVQQLVDLLCRLELYRAAQIILNWKPAPEIRCPIPAFPDSVKPEKPLAASVRKAEDEQEEGQPVRMATFPGPGSSPARAHQPAFLQPPEEDAPHSLRSDLPTSSDSKDFSTSIPKQEKLLSLAGDSLFWSEADVVQATDDFNQNRKISQGTFADVYRGHRHGKPFVFKKLRETACSSPGSI Gene ID Swiss Prot Synonyms IRAK-2 Calculated MW 69kDa Observed MW 70kDa Applications
Reactivity Human, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HeLa, HepG2, K-562 Cellular location Cytoplasm, Cytosol, Nucleus, Plasma membrane 
Western blot analysis of extracts of various cell lines, using IRAK2 Rabbit mAb (A4655) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded rat kidney using IRAK2 Rabbit mAb (A4655) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 50-250 of human IRAK2 (O43187). Isotype IgG