быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
TCP1 beta/CCT2 Rabbit mAb (A4700)
- Описание
- Характеристики
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Overview
Product name TCP1 beta/CCT2 Rabbit mAb Catalog No. A4700 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1097 Background
The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 436-535 of human TCP1 beta/CCT2 (P78371). Sequence MESYAKALRMLPTIIADNAGYDSADLVAQLRAAHSEGNTTAGLDMREGTIGDMAILGITESFQVKRQVLLSAAEAAEVILRVDNIIKAAPRKRVPDHHPC Gene ID Swiss Prot Synonyms CCTB; 99D8.1; PRO1633; CCT-beta; HEL-S-100n; TCP-1-beta Calculated MW 57kDa Observed MW 57kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:2000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HeLa, Raji, SH-SY5Y, Mouse liver, Mouse testis, Mouse brain, Rat brain Cellular location Cytoplasm 
Western blot analysis of extracts of various cell lines, using TCP1 beta/CCT2 Rabbit mAb (A4700) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded rat brain using TCP1 beta/CCT2 Rabbit mAb (A4700) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 436-535 of human TCP1 beta/CCT2 (P78371). Isotype IgG