быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Galectin 1/LGALS1 Rabbit mAb (A4732)
- Описание
- Характеристики
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Overview
Product name Galectin 1/LGALS1 Rabbit mAb Catalog No. A4732 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1110 Background
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Galectin 1/LGALS1 (P09382). Sequence MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVK Gene ID Swiss Prot Synonyms GBP; GAL1 Calculated MW 15kDa Observed MW 14kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples U2OS, A-549, HL-60, Mouse testis, Mouse thymus, Rat lung, Rat heart Cellular location Secreted, extracellular matrix, extracellular space 
Western blot analysis of extracts of various cell lines, using Galectin 1/LGALS1 Rabbit mAb (A4732) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Galectin 1/LGALS1 Rabbit mAb (A4732) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH-3T3 cells using Galectin 1/LGALS1 Rabbit mAb (A4732) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Galectin 1/LGALS1 (P09382). Isotype IgG