быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
KIF23 Rabbit mAb (A4896)
- Описание
- Характеристики
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Overview
Product name KIF23 Rabbit mAb Catalog No. A4896 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0335 Background
The protein encoded by this gene is a member of kinesin-like protein family. This family includes microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein has been shown to cross-bridge antiparallel microtubules and drive microtubule movement in vitro. Alternate splicing of this gene results in multiple transcript variants.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 801-960 of human KIF23 (Q02241). Sequence NAPPIRLRHRRSRSAGDRWVDHKPASNMQTETVMQPHVPHAITVSVANEKALAKCEKYMLTHQELASDGEIETKLIKGDIYKTRGGGQSVQFTDIETLKQESPNGSRKRRSSTVAPAQPDGAESEWTDVETRCSVAVEMRAGSQLGPGYQHHAQPKRKKP Gene ID Swiss Prot Synonyms CDA3; CHO1; CDAN3; KNSL5; MKLP1; CDAIII; CDAN3A; MKLP-1 Calculated MW 110kDa Observed MW 115kDa Applications
Reactivity Human, Rat Tested applications WB IP Recommended dilution - WB 1:500 - 1:1000
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunoprecipitation Positive samples HeLa, Rat testis Cellular location Cytoplasm, Midbody, Nucleus, cytoskeleton, spindle 
Western blot analysis of extracts of various cell lines, using KIF23 Rabbit mAb (A4896) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg KIF23 antibody (A4896). Western blot was performed from the immunoprecipitate using KIF23 antibody (A4896) at a dilution of 1:1000.
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Key applications Western blotting, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 801-960 of human KIF23 (Q02241). Isotype IgG