быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Hsp90α Rabbit mAb (A5006)
- Описание
- Характеристики
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Overview
Product name Hsp90α Rabbit mAb Catalog No. A5006 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1167 Background
The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Hsp90α (NP_005339.3). Sequence MPEETQTQDQPMEEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKLDSGKELHINLIPNKQDRTLTIVDTGIGMTK Gene ID Swiss Prot Synonyms EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p Calculated MW 85kDa Observed MW 90kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IP Recommended dilution - WB 1:500 - 1:2000
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunoprecipitation Positive samples HeLa, Raji, Mouse brain, Rat brain, 293T Cellular location Cell membrane, Cytoplasm, Melanosome Customer validation ( 293T HelaEGF HepG2 A549 Fuov-1 扁桃体 Jurket )
Western Blot(Mus musculus)
WB(Homo sapiens)
IF(Homo sapiens,Mus musculus)
Western blot analysis of extracts of various cell lines, using Hsp90α Rabbit mAb (A5006) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg Hsp90α antibody (A5006). Western blot was performed from the immunoprecipitate using Hsp90α antibody (A5006) at a dilution of 1:1000.
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Key applications Western blotting, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Hsp90α (NP_005339.3). Isotype IgG