быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Hsp90α/β Rabbit mAb (A5027)
- Описание
- Характеристики
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Overview
Product name Hsp90α/β Rabbit mAb Catalog No. A5027 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1169 Background
The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 533-732 of human Hsp90α (P07900). Sequence LKEFEGKTLVSVTKEGLELPEDEEEKKKQEEKKTKFENLCKIMKDILEKKVEKVVVSNRLVTSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMAAKKHLEINPDHSIIETLRQKAEADKNDKSVKDLVILLYETALLSSGFSLEDPQTHANRIYRMIKLGLGIDEDDPTADDTSAAVTEEMPPLEGDDDTSRMEEVD Gene ID Swiss Prot Synonyms EL52; HEL-S-65p; HSP86; HSP89A; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp103; Hsp89; Hsp90; LAP-2; LAP2 Calculated MW 90kDa Observed MW 90kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, Raji, Mouse liver, Rat liver, Rat brain Cellular location Cell membrane, Cytoplasm, Melanosome Customer validation WB(Mus musculus, Homo sapiens, Gallus gallus)
WB，IHC，IF，ELISA(Mus musculus)
Western blot analysis of extracts of various cell lines, using Hsp90α/β Rabbit mAb (A5027) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 533-732 of human Hsp90α (P07900). Isotype IgG