быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
STAT5 Rabbit mAb (A5029)
- Описание
- Характеристики
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Overview
Product name STAT5 Rabbit mAb Catalog No. A5029 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1215 Background
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 600-786 of human STAT5 (P42229). Sequence KQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSPERNLWNLKPFTTRDFSIRSLADRLGDLSYLIYVFPDRPKDEVFSKYYTPVLAKAVDGYVKPQIKQVVPEFVNASADAGGSSATYMDQAPSPAVCPQAPYNMYPQNPDHVLDQDGEFDLDETMDVARHVEELLRRPMDSLDSRLSPPAGLF Gene ID Swiss Prot Synonyms MGF; STAT5 Calculated MW 90kDa Observed MW 90kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, PC-3, Mouse heart, Rat lung Cellular location Cytoplasm, Nucleus Customer validation (293T、MOLT-4、T47D、HeLa、A549、HepG2、MCF7、RPMI-8226)
WB(Mus musculus, Homo sapiens)
FC(Mus musculus)
Western blot analysis of extracts of various cell lines, using STAT5 Rabbit mAb (A5029) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
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Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 600-786 of human STAT5 (P42229). Isotype IgG