быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
MAD1/MAD1L1 Rabbit mAb (A5098)
- Описание
- Характеристики
-
Overview
Product name MAD1/MAD1L1 Rabbit mAb Catalog No. A5098 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1188 Background
MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 50-150 of human MAD1/MAD1L1 (Q9Y6D9). Sequence QLEERAEQIRSKSHLIQVEREKMQMELSHKRARVELERAASTSARNYEREVDRNQELLTRIRQLQEREAGAEEKMQEQLERNRQCQQNLDAASKRLREKED Gene ID Swiss Prot Synonyms MAD1; MVA7; PIG9; TP53I9; TXBP181 Calculated MW 83kDa Observed MW 83kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, 293T, Mouse spleen Cellular location Chromosome, Cytoplasm, Nucleus, Centromere, Centrosome, Cytoskeleton, Kinetochore, Microtubule organizing center, Spindle 
Western blot analysis of extracts of Mouse spleen, using MAD1/MAD1L1 Rabbit mAb (A5098) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Western blot analysis of extracts of various cell lines, using MAD1/MAD1L1 Rabbit mAb (A5098) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen A synthetic peptide corresponding to a sequence within amino acids 50-150 of human MAD1/MAD1L1 (Q9Y6D9). Isotype IgG