быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Desmoglein 3 Rabbit mAb (A5227)
- Описание
- Характеристики
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Overview
Product name Desmoglein 3 Rabbit mAb Catalog No. A5227 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1190 Background
This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. The encoded protein has been identified as the autoantigen of the autoimmune blistering disease pemphigus vulgaris.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 900-999 of human Desmoglein 3 (P32926). Sequence TLSGSQGASALSTSGSVQPAVSIPDPLQHGNYLVTETYSASGSLVQPSTAGFDPLLTQNVIVTERVICPISSVPGNLAGPTQLRGSHTMLCTEDPCSRLI Gene ID Swiss Prot Synonyms PVA; ABOLM; CDHF6 Calculated MW 108kDa Observed MW 145kDa Applications
Reactivity Human, Mouse Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples A-431, Cal-27 Cellular location Cell junction, Cell membrane, Single-pass type I membrane protein, desmosome 
Western blot analysis of extracts of various cell lines, using Desmoglein 3 Rabbit mAb (A5227) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
Immunofluorescence analysis of NIH-3T3 cells using Desmoglein 3 Rabbit mAb (A5227) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen A synthetic peptide corresponding to a sequence within amino acids 900-999 of human Desmoglein 3 (P32926). Isotype IgG