быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
p150 CAF1 Rabbit mAb (A8725)
- Описание
- Характеристики
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Overview
Product name p150 CAF1 Rabbit mAb Catalog No. A8725 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2011 Background
Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 100-200 of human p150 CAF1 (NP_005474.2). Sequence NFLRNRIETSIGQSTVIIDLTEDSNEQPDSLVDHNKLNSEASPSREAINGQREDTGDQQGLLKAIQNDKLAFPGETLSDIPCKTEEEGVGCGGAGRRGDSQ Gene ID Swiss Prot Synonyms CAF1; P150; CAF-1; CAF1B; CAF1P150 Calculated MW 107kDa Observed MW 150kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, Jurkat, SH-SY5Y Cellular location Nucleus 
Western blot analysis of extracts of various cell lines, using p150 CAF1 Rabbit mAb (A8725) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthetic peptide corresponding to a sequence within amino acids 100-200 of human p150 CAF1 (NP_005474.2). Isotype IgG