быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
PELP1 Rabbit mAb (A9026)
- Описание
- Характеристики
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Overview
Product name PELP1 Rabbit mAb Catalog No. A9026 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1387 Background
This gene encodes a transcription factor which coactivates transcription of estrogen receptor responsive genes and corepresses genes activated by other hormone receptors or sequence-specific transcription factors. Expression of this gene is regulated by both members of the estrogen receptor family. This gene may be involved in the progression of several types of cancer. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1000-1100 of human PELP1 (Q8IZL8). Sequence EPEPGLLLEVEEPGTEEERGADTAPTLAPEALPSQGEVEREGESPAAGPPPQELVEEEPSAPPTLLEEETEDGSDKVQPPPETPAEEEMETETEAEALQEK Gene ID Swiss Prot Synonyms MNAR; P160 Calculated MW 120kDa Observed MW 160kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples SW620, MCF7, SKOV3, NIH/3T3, Rat testis Cellular location Cytoplasm, Nucleus, nucleoplasm 
Western blot analysis of extracts of various cell lines, using PELP1 Rabbit mAb (A9026) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts of Rat testis, using PELP1 Rabbit mAb (A9026) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1000-1100 of human PELP1 (Q8IZL8). Isotype IgG