быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Fyn Rabbit mAb (A9165)
- Описание
- Характеристики
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Overview
Product name Fyn Rabbit mAb Catalog No. A9165 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1459 Background
This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Fyn (P06241). Sequence MGCVQCKDKEATKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDD Gene ID Swiss Prot Synonyms SLK; SYN; p59-FYN Calculated MW 61kDa Observed MW 59kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, Jurkat, Mouse lung, Mouse testis, Mouse brain, Rat brain Cellular location Cell membrane, Cytoplasm, Nucleus 
Western blot analysis of extracts of various cell lines, using Fyn Rabbit mAb (A9165) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Fyn (P06241). Isotype IgG