быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Fascin/FSCN1 Rabbit mAb (A9566)
- Описание
- Характеристики
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Overview
Product name Fascin/FSCN1 Rabbit mAb Catalog No. A9566 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1638 Background
This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 249-362 of human Fascin/FSCN1 (Q16658). Sequence GKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKNGQ Gene ID Swiss Prot Synonyms HSN; SNL; p55; FAN1 Calculated MW 55kDa Observed MW 54kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HeLa, HepG2, Mouse brain, Mouse kidney, Mouse heart, Rat brain Cellular location Cell junction, Cell projection, Cytoplasm, cytoskeleton, cytosol, filopodium, invadopodium, microvillus 
Western blot analysis of extracts of various cell lines, using Fascin/FSCN1 Rabbit mAb (A9566) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded Human anaplastic large cell lymphoma using Fascin/FSCN1 Rabbit mAb (A9566) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human Hodgkin lymphoma using Fascin/FSCN1 Rabbit mAb (A9566) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human tonsil using Fascin/FSCN1 Rabbit mAb (A9566) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 249-362 of human Fascin/FSCN1 (Q16658). KO Validated Validated Isotype IgG