быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Apolipoprotein CI Rabbit mAb (A9570)
- Описание
- Характеристики
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Overview
Product name Apolipoprotein CI Rabbit mAb Catalog No. A9570 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1641 Background
This gene encodes a member of the apolipoprotein C1 family. This gene is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. The encoded protein plays a central role in high density lipoprotein (HDL) and very low density lipoprotein (VLDL) metabolism. This protein has also been shown to inhibit cholesteryl ester transfer protein in plasma. A pseudogene of this gene is located 4 kb downstream in the same orientation, on the same chromosome. This gene is mapped to chromosome 19, where it resides within a apolipoprotein gene cluster. Alternative splicing and the use of alternative promoters results in multiple transcript variants.Immunogen information
Immunogen A synthesized peptide derived from human APOC1(P02654). Sequence Email for sequence Gene ID Swiss Prot Synonyms Apo-CI; ApoC-I; apo-CIB; apoC-IB Calculated MW 9kDa Observed MW 8kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples Human plasma Cellular location endoplasmic reticulum, extracellular region 
Western blot analysis of extracts of human plasma, using (A9570) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human placenta using Apolipoprotein CI Rabbit mAb (A9570) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of rat liver using Apolipoprotein CI Rabbit mAb (A9570) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of human liver using Apolipoprotein CI Rabbit mAb (A9570) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse liver using Apolipoprotein CI Rabbit mAb (A9570) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthesized peptide derived from human APOC1(P02654). Isotype IgG