быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CDC34 Rabbit mAb (A9772)
- Описание
- Характеристики
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Overview
Product name CDC34 Rabbit mAb Catalog No. A9772 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1742 Background
The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 20-236 of human CDC34 (P49427). Sequence LQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES Gene ID Swiss Prot Synonyms UBC3; UBCH3; UBE2R1; E2-CDC34 Calculated MW 27kDa Observed MW 32kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples Jurkat, SH-SY5Y Cellular location Cytoplasm, Nucleus 
Western blot analysis of extracts of various cell lines, using CDC34 Rabbit mAb (A9772) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 20-236 of human CDC34 (P49427). Isotype IgG