быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Cytokeratin 1 (KRT1) Rabbit mAb (A9776)
- Описание
- Характеристики
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Overview
Product name Cytokeratin 1 (KRT1) Rabbit mAb Catalog No. A9776 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1745 Background
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 545-644 of human Cytokeratin 1 (KRT1) (P04264). Sequence GGGGRGSYGSGGSSYGSGGGSYGSGGGGGGHGSYGSGSSSGGYRGGSGGGGGGSSGGRGSGGGSSGGSIGGRGSSSGGVKSSGGSSSVKFVSTTYSGVTR Gene ID Swiss Prot Synonyms K1; CK1; EHK; AEI2; EHK1; EPPK; KRT1A; NEPPK Calculated MW 66kDa Observed MW 70kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples Mouse brain, Rat brain, A375 Cellular location Cell membrane 
Western blot analysis of extracts of various cell lines, using Cytokeratin 1 (KRT1) (KRT1) Rabbit mAb (A9776) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts of A375 cells, using Cytokeratin 1 (KRT1) (KRT1) Rabbit mAb (A9776) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded rat skin using Cytokeratin 1 (KRT1) (KRT1) Rabbit mAb (A9776) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Cytokeratin 1 (KRT1) (KRT1) Rabbit mAb (A9776) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse skin using Cytokeratin 1 (KRT1) (KRT1) Rabbit mAb (A9776) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of rat skin using Cytokeratin 1 (KRT1) (KRT1) Rabbit mAb (A9776) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of human skin using Cytokeratin 1 (KRT1) (KRT1) Rabbit mAb (A9776) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Mouse tail and ear using Cytokeratin 1 (KRT1) (KRT1) Rabbit mAb (A9776) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 545-644 of human Cytokeratin 1 (KRT1) (P04264). Isotype IgG