быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Cytokeratin 10 Rabbit mAb (A4669)
- Описание
- Характеристики
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Overview
Product name Cytokeratin 10 Rabbit mAb Catalog No. A4669 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1084 Background
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 485-584 of human Cytokeratin 10 (P13645). Sequence GGHGGGHGGSSGGGYGGGSSGGGSSGGGYGGGSSSGGHGGSSSGGYGGGSSGGGGGGYGGGSSGGGSSSGGGYGGGSSSGGHKSSSSGSVGESSSKGPRY Gene ID Swiss Prot Synonyms BIE; EHK; IHL; K10; KPP; BCIE; CK10 Calculated MW 59kDa Observed MW 60kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:100 - 1:500
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa, A-431 Cellular location cell surface, cytoplasm, cytoskeleton, cytosol, extracellular exosome, extracellular space, nucleus Customer validation IF(Homo sapiens)
Western blot analysis of extracts of various cell lines, using Cytokeratin 10 antibody (A4669) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunofluorescence analysis of rat skin using Cytokeratin 10 Rabbit mAb (A4669) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of human skin using Cytokeratin 10 Rabbit mAb (A4669) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Mouse tail and ear using Cytokeratin 10 Rabbit mAb (A4669) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 485-584 of human Cytokeratin 10 (P13645). Isotype IgG