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Размер
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KAT13A / SRC1 Rabbit mAb (A9058)
- Описание
- Характеристики
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Overview
Product name KAT13A / SRC1 Rabbit mAb Catalog No. A9058 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1395 Background
The protein encoded by this gene acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. The product of this gene binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion. Alternatively spliced transcript variants encoding different isoforms have been identified.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 400-750 of human KAT13A / SRC1 (Q15788). Sequence VARSSTLPPSNSNMVSTRINRQQSSDLHSSSHSNSSNSQGSFGCSPGSQIVANVALNQGQASSQSSNPSLNLNNSPMEGTGISLAQFMSPRRQVTSGLATRPRMPNNSFPPNISTLSSPVGMTSSACNNNNRSYSNIPVTSLQGMNEGPNNSVGFSASSPVLRQMSSQNSPSRLNIQPAKAESKDNKEIASILNEMIQSDNSSSDGKPLDSGLLHNNDRLSDGDSKYSQTSHKLVQLLTTTAEQQLRHADIDTSCKDVLSCTGTSNSASANSSGGSCPSSHSSLTERHKILHRLLQEGSPSDITTLSVEPDKKDSASTSVSVTGQVQGNSSIKLELDASKKKESKDHQLLR Gene ID Swiss Prot Synonyms SRC1; KAT13A; RIP160; F-SRC-1; bHLHe42; bHLHe74 Calculated MW 157kDa Observed MW 180kDa Applications
Reactivity Human Tested applications WB IP Recommended dilution - WB 1:500 - 1:1000
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunoprecipitation Positive samples HeLa, 293T, SGC-7901 Cellular location cytosol, nucleoplasm, nucleus, plasma membrane 
Western blot analysis of extracts of various cell lines, using KAT13A / SRC1 antibody (A9058) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg KAT13A/SRC1 antibody (A9058). Western blot was performed from the immunoprecipitate using KAT13A/SRC1 antibody (A9058) at a dilution of 1:1000.
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Key applications Western blotting, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 400-750 of human KAT13A / SRC1 (Q15788). Isotype IgG