быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
mSin3A Rabbit mAb (A2396)
- Описание
- Характеристики
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Overview
Product name mSin3A Rabbit mAb Catalog No. A2396 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0745 Background
The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1174-1273 of human mSin3A (Q96ST3). Sequence YKMVYVIKSEDYMYRRTALLRAHQSHERVSKRLHQRFQAWVDKWTKEHVPREMAAETSKWLMGEGLEGLVPCTTTCDTETLHFVSINKYRVKYGTVFKAP Gene ID Swiss Prot Synonyms WITKOS; DEL15Q24; CHR15DELq24 Calculated MW 145kDa Observed MW 145-160kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples Mouse testis, Rat testis Cellular location histone deacetylase complex, nucleolus, nucleoplasm, nucleus 
Western blot analysis of extracts of various cell lines, using mSin3A antibody (A2396) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded rat testis using mSin3A Rabbit mAb (A2396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon using mSin3A Rabbit mAb (A2396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using mSin3A Rabbit mAb (A2396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH-3T3 cells using mSin3A Rabbit mAb (A2396) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using mSin3A Rabbit mAb (A2396) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1174-1273 of human mSin3A (Q96ST3). Isotype IgG