быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
MT-ND1 Rabbit mAb (A9743)
- Описание
- Характеристики
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Overview
Product name MT-ND1 Rabbit mAb Catalog No. A9743 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1728 Background
Enables NADH dehydrogenase (ubiquinone) activity. Involved in mitochondrial electron transport, NADH to ubiquinone and mitochondrial respiratory chain complex I assembly. Located in mitochondrial membrane. Part of mitochondrial respiratory chain complex I. Implicated in several diseases, including MELAS syndrome; neurodegenerative disease (multiple); optic nerve disease (multiple); toxic shock syndrome; and type 2 diabetes mellitus. Biomarker of Alzheimer's disease; Parkinson's disease; and multiple sclerosis.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MT-ND1 (P03886). Sequence MPMANLLLLIVPILIAMAFLMLTERKILGYMQLRKGPNVVGPYGLLQPFADAMKLFTKEPLKPATSTITLYITAPTLALTIALLLWTPLPMPNPLVNLNL Gene ID Swiss Prot Synonyms MTND1; ND1 Calculated MW 36kDa Observed MW 35kDa Applications
Reactivity Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples Mouse kidney, Rat brain, Rat kidney Cellular location mitochondrial inner membrane, mitochondrial membrane, mitochondrial respiratory chain complex I 
Western blot analysis of extracts of various cell lines, using MT-ND1 antibody (A9743) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MT-ND1 (P03886). Isotype IgG