быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
CD147/BSG Rabbit mAb (A4310)
- Описание
- Характеристики
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Overview
Product name CD147/BSG Rabbit mAb Catalog No. A4310 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0964 Background
The protein encoded by this gene, basigin, is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. Basigin is also a member of the immunoglobulin superfamily, ubiquitously expressed in various tissues. Multiple transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CD147/BSG (P35613). Sequence EEDTGTYECRASNDPDRNHLTRAPRVKWVRAQAVVLVLEPGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGE Gene ID Swiss Prot Synonyms OK; 5F7; TCSF; CD147; EMPRIN; HAb18G; EMMPRIN Calculated MW 19kDa/22kDa/29kDa/42kDa Observed MW 40-60kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T, U-251MG, Mouse liver, Mouse brain, Mouse heart Cellular location Cell membrane, Melanosome, Single-pass type I membrane protein 
Western blot analysis of various lysates, using CD147/BSG antibody (A4310) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CD147/BSG (P35613). Isotype IgG