быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
EG5/KIF11 Rabbit mAb (A4659)
- Описание
- Характеристики
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Overview
Product name EG5/KIF11 Rabbit mAb Catalog No. A4659 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1080 Background
This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 900-1056 of human EG5/KIF11 (P52732). Sequence ETIKIGLTKLNCFLEQDLKLDIPTGTTPQRKSYLYPSTLVRTEPREHLLDQLKRKQPELLMMLNCSENNKEETIPDVDVEEAVLGQYTEEPLSQEPSVDAGVDCSSIGGVPFFQHKKSHGKDKENRGINTLERSKVEETTEHLVTKSRLPLRAQINL Gene ID Swiss Prot Synonyms EG5; HKSP; KNSL1; MCLMR; TRIP5 Calculated MW 119kDa Observed MW 130kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples A549, MCF7 Cellular location Cytoplasm, cytoskeleton 
Western blot analysis of extracts of various cell lines, using EG5/KIF11 Rabbit mAb (A4659) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 900-1056 of human EG5/KIF11 (P52732). Isotype IgG