быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Cullin 2 Rabbit mAb (A5076)
- Описание
- Характеристики
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Overview
Product name Cullin 2 Rabbit mAb Catalog No. A5076 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1248 Background
Enables ubiquitin protein ligase binding activity. Predicted to be involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process and protein ubiquitination. Predicted to act upstream of or within protein catabolic process. Located in nucleoplasm. Part of Cul2-RING ubiquitin ligase complex.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cullin 2 (Q13617). Sequence MSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSKGADY Gene ID Swiss Prot Synonyms CUL2; cullin-2 Calculated MW 87kDa/89kDa Observed MW 87kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HeLa, Mouse liver, Rat testis, Rat brain Cellular location Cytosol, Nucleolus, Nucleoplasm 
Western blot analysis of extracts of various cell lines, using Cullin 2 Rabbit mAb (A5076) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded human appendix using Cullin 2 Rabbit mAb (A5076) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cullin 2 (Q13617). Isotype IgG